lundi 3 décembre 2012
vendredi 28 septembre 2012
La SNCF condamnée après la mort d'un agent atteint de légionellose - Justice - France Info
La SNCF condamnée après la mort d'un agent atteint de légionellose - Justice - France Info
Le département R&D de CEERAM a développé une méthode de génotypage de différentes bactéries par MLVA. A l’instar des techniques utilisées par la médecine légale pour l’élaboration d’empreintes génétiques humaines, cette méthode d'analyse s’appuie sur l’étude concomitante du polymorphisme de plusieurs VNTRs, séquences répétées en tandem caractérisée par une variabilité de répétition intra-espèce.
La plate-forme de génotypage bactérien dont dispose CEERAM vous permettra de typer systématiquement tous les isolats collectés afin que des décisions puissent être rapidement prises lors de cas épidémiques, ou que certaines mesures préventives soient engagées.
Ce cheminot avait contracté la légionellose diffusée par une tour aéroréfrigérante de la gare d'Austerlitz à Paris, avant de décéder fin août 2006. Condamnée pour "homicide involontaire", la SNCF devra payer 50.000 euros d'amende.
| ____________________________________________________ |
Une plate-forme de génotypage bactérien
Le département R&D de CEERAM a développé une méthode de génotypage de différentes bactéries par MLVA. A l’instar des techniques utilisées par la médecine légale pour l’élaboration d’empreintes génétiques humaines, cette méthode d'analyse s’appuie sur l’étude concomitante du polymorphisme de plusieurs VNTRs, séquences répétées en tandem caractérisée par une variabilité de répétition intra-espèce.
Pourquoi choisir l’expertise CEERAM en génotypage bactérien?
- Typage de 100 souches garanti en 72 heures
- Validée sur souches cliniques et environnementales
- Résultats reproductibles
- Pouvoir de discrimination élevé
- Code MLVA numérique échangeable et stockable
- Création d’une base de données
- Méthodes brevetées (S. aureus, L. pneumophila)
La plate-forme de génotypage bactérien dont dispose CEERAM vous permettra de typer systématiquement tous les isolats collectés afin que des décisions puissent être rapidement prises lors de cas épidémiques, ou que certaines mesures préventives soient engagées.
Pourquoi choisir les kits MLVA ceeramTools™ de génotypage bactérien?
- Une solution prête à l'emploi pour une utilisation au sein de votre propre laboratoire
- Amplification efficace
- Données traitées par GeneMapper®
- Adaptés aux séquenceurs ABI
- Utilisés par des Centres de Référence européens.
Les bactéries typées par méthode MLVA (Multi-Locus-VNTR-Analysis)?
-
Pseudomonas aeruginosa
-
Legionella pneumophila
-
Staphylococcus aureus
-
Salmonella enterica
mardi 28 août 2012
Legionnaires' disease outbreak kills 2 at Chicago hotel - Legionella pneumophila outbreak
Legionnaires' disease outbreak kills 2 at Chicago hotel - CNN.com
A Chicago hotel has closed and drained its pool, hot tub and fountain in response to the deaths of two people who contracted the flu-like Legionnaires' disease, authorities said Monday.
_______________________________
Genotyping Legionella pneumophila
Reveal outbreak cases to initiate community containment measures.
Determine the source of the contamination (hot water system or cooling tower)
Trace the spread and source of the pathogen during nosocomial outbreaks.
Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
Characterize a travel-acquired legionellosis.
Emphasize the presence/emergence of virulent strains/clones (Paris, Lorraine,…)
Study L. pneumophila epidemiology to know its evolution and evaluate emergence conditions.
Multi Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for L. pneumophila examines 12 loci. Thus, each isolate is defined by a 12-digit numeric code corresponding to the number of repeats at each VNTR.
Efficient amplification of the 12 markers in a single PCR. Required equipment: ABI sequencer to analyze the amplicons. The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code. Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.
Our Expertise Department has an automatized and standardized genotyping platform at disposal. The procedure involves three steps:
Download our brochure
A Chicago hotel has closed and drained its pool, hot tub and fountain in response to the deaths of two people who contracted the flu-like Legionnaires' disease, authorities said Monday.
_______________________________
Legionella pneumophila
Genotyping Legionella pneumophila
Overview of L. pneumophila
L. pneumophila is the main etiologic agent of legionellosis, a severe respiratory disease transmitted by inhalation of aqueous aerosols. L. pneumophila is an ubiquitous aquatic bacteria but also has a widespread distribution in man-made environments. Most legionellosis outbreaks are linked to contaminated hot water systems or cooling towers. Due to L. pneumophila large occurrence in both natural and artificial aquatic systems, Ceeram has been developing a powerful typing method to allow the implementation of prevention measures, to identify potential environmental sources of infection by comparing clinical and environmental isolatesWhat is Bacterial Typing?
A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.Why typing L. pneumophila
Reveal outbreak cases to initiate community containment measures.
Determine the source of the contamination (hot water system or cooling tower)
Trace the spread and source of the pathogen during nosocomial outbreaks.
Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
Characterize a travel-acquired legionellosis.
Emphasize the presence/emergence of virulent strains/clones (Paris, Lorraine,…)
Study L. pneumophila epidemiology to know its evolution and evaluate emergence conditions.
MLVA, a powerful typing method
Multi Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for L. pneumophila examines 12 loci. Thus, each isolate is defined by a 12-digit numeric code corresponding to the number of repeats at each VNTR.
Genotyping Kit
Efficient amplification of the 12 markers in a single PCR. Required equipment: ABI sequencer to analyze the amplicons. The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code. Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.
Ceeram Solution : Service
Our Expertise Department has an automatized and standardized genotyping platform at disposal. The procedure involves three steps:
- Samples receipt (DNA or colonies on plates)
- MLVA genotyping and
- Analysis report (isolates relatedness).
Download our brochure
mardi 21 août 2012
Three dead in Quebec City after outbreak of Legionnaires' disease
Three dead in Quebec City after outbreak of Legionnaires' disease
An outbreak of Legionnaires' disease has claimed three lives in the Quebec City region, prompting public health authorities to order the immediate inspection of cooling towers in office buildings - believed to be the source of the infections.
An outbreak of Legionnaires' disease has claimed three lives in the Quebec City region, prompting public health authorities to order the immediate inspection of cooling towers in office buildings - believed to be the source of the infections.
lundi 11 juin 2012
Deadly disease outbreak in Scotland - Legionellosis
Deadly disease outbreak in Scotland - World - NZ Herald News
It means 36 people have now been struck down by the bug which has already caused the death of one man, with a further 44 in Edinburgh waiting for test results confirming whether they are also infected with the waterborne virus.
Of the 80 confirmed and suspected cases, 15 are in intensive care and 27 are in general wards. A further 16 cases are being treated in the community and a number of people have already been discharged from hospital.
_______________________________________________________________
Download our brochure
It means 36 people have now been struck down by the bug which has already caused the death of one man, with a further 44 in Edinburgh waiting for test results confirming whether they are also infected with the waterborne virus.
Of the 80 confirmed and suspected cases, 15 are in intensive care and 27 are in general wards. A further 16 cases are being treated in the community and a number of people have already been discharged from hospital.
_______________________________________________________________
Genotyping Legionella pneumophila |  |  |  |
Overview of L. pneumophila
L. pneumophila is the main etiologic agent of legionellosis, a severe respiratory disease transmitted by inhalation of aqueous aerosols. L. pneumophila is an ubiquitous aquatic bacteria but also has a widespread distribution in man-made environments. Most legionellosis outbreaks are linked to contaminated hot water systems or cooling towers. Due to L. pneumophila large occurrence in both natural and artificial aquatic systems, Ceeram has been developing a powerful typing method to allow the implementation of prevention measures, to identify potential environmental sources of infection by comparing clinical and environmental isolates
What is Bacterial Typing?
A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.
Why typing L. pneumophila
- Reveal outbreak cases to initiate community containment measures.
- Determine the source of the contamination (hot water system or cooling tower)
- Trace the spread and source of the pathogen during nosocomial outbreaks.
- Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
- Characterize a travel-acquired legionellosis.
- Emphasize the presence/emergence of virulent strains/clones (Paris, Lorraine,…)
- Study L. pneumophila epidemiology to know its evolution and evaluate emergence conditions.
MLVA, a powerful typing method
Multi Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for L. pneumophila examines 12 loci. Thus, each isolate is defined by a 12-digit numeric code corresponding to the number of repeats at each VNTR.
Genotyping Kit
Efficient amplification of the 12 markers in a single PCR. Required equipment: ABI sequencer to analyze the amplicons. The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code. Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.
Ceeram Solution : Service
Our Expertise Department has an automatized and standardized genotyping platform at disposal. The procedure involves three steps:
- Samples receipt (DNA or colonies on plates)
- MLVA genotyping and
- Analysis report (isolates relatedness).
Download our brochure
Legionnaires outbreak: Eight new confirmed cases takes total to 80 - The Daily Record
Legionnaires outbreak: Eight new confirmed cases takes total to 80 - The Daily Record
There are now 80 confirmed and suspected cases of Legionnaires' disease from the outbreak in the south-west of Edinburgh.
_____________________
Download our brochure
There are now 80 confirmed and suspected cases of Legionnaires' disease from the outbreak in the south-west of Edinburgh.
_____________________
Genotyping Legionella pneumophila | | | |
Overview of L. pneumophila
L. pneumophila is the main etiologic agent of legionellosis, a severe respiratory disease transmitted by inhalation of aqueous aerosols. L. pneumophila is an ubiquitous aquatic bacteria but also has a widespread distribution in man-made environments. Most legionellosis outbreaks are linked to contaminated hot water systems or cooling towers. Due to L. pneumophila large occurrence in both natural and artificial aquatic systems, Ceeram has been developing a powerful typing method to allow the implementation of prevention measures, to identify potential environmental sources of infection by comparing clinical and environmental isolates
What is Bacterial Typing?
A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.
Why typing L. pneumophila
- Reveal outbreak cases to initiate community containment measures.
- Determine the source of the contamination (hot water system or cooling tower)
- Trace the spread and source of the pathogen during nosocomial outbreaks.
- Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
- Characterize a travel-acquired legionellosis.
- Emphasize the presence/emergence of virulent strains/clones (Paris, Lorraine,…)
- Study L. pneumophila epidemiology to know its evolution and evaluate emergence conditions.
MLVA, a powerful typing method
Multi Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for L. pneumophila examines 12 loci. Thus, each isolate is defined by a 12-digit numeric code corresponding to the number of repeats at each VNTR.
Genotyping Kit
Efficient amplification of the 12 markers in a single PCR. Required equipment: ABI sequencer to analyze the amplicons. The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code. Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.
Ceeram Solution : Service
Our Expertise Department has an automatized and standardized genotyping platform at disposal. The procedure involves three steps:
- Samples receipt (DNA or colonies on plates)
- MLVA genotyping and
- Analysis report (isolates relatedness).
Download our brochure
vendredi 8 juin 2012
Managing Norovirus waste | Blenkharn Environmental
Managing Norovirus waste | Blenkharn Environmental
http://www.ianblenkharn.com/?p=3621
Norovirus outbreaks continue to be a major seasonal problem and cause huge disruption to care services with substantial morbidity and mortality. What to do with Norovirus waste?
Much waste will be destined for the toilet or bedpan washer/macerator and though this creates a substantial risk of further aerosol distribution that is a matter for another day.
http://www.ianblenkharn.com/?p=3621
Norovirus outbreaks continue to be a major seasonal problem and cause huge disruption to care services with substantial morbidity and mortality. What to do with Norovirus waste?Much waste will be destined for the toilet or bedpan washer/macerator and though this creates a substantial risk of further aerosol distribution that is a matter for another day.
The Hunt for the Norovirus
The Hunt for the Norovirus
This virus is the source of an estimated 20 million, at a minimum, cases of gastroenteritis each year in the US. More than 70,000 hospital admissions and even 800 plus fatalities. They are robust microbes that survive for long periods, invisibly, on the surfaces of everyday objects. While the chances of catching the norovirus from a reusable bag is very slight be aware that a 2010 study did find huge numbers of pathogenic bacteria in and on reusable grocery bags. Most notably twelve percent of them were found to harbor e-coli.
More at : http://www.hivehealthmedia.com/hunt-norovirus/
This virus is the source of an estimated 20 million, at a minimum, cases of gastroenteritis each year in the US. More than 70,000 hospital admissions and even 800 plus fatalities. They are robust microbes that survive for long periods, invisibly, on the surfaces of everyday objects. While the chances of catching the norovirus from a reusable bag is very slight be aware that a 2010 study did find huge numbers of pathogenic bacteria in and on reusable grocery bags. Most notably twelve percent of them were found to harbor e-coli.
More at : http://www.hivehealthmedia.com/hunt-norovirus/
mardi 5 juin 2012
Evita intoxicaciones, ¡lava tus verduras! | Telemundo 47 - Nueva York
Evita intoxicaciones, ¡lava tus verduras! | Telemundo 47 - Nueva York
Agentes patógenos como el E. Coli, Salmonela, Listeria o el Norovirus pueden afectar a los alimentos durante su cosecha o proceso de manipulación. El hecho de que los vegetales provengan de una granja orgánica no es suficiente para descartar la posibilidad de contaminación.
Agentes patógenos como el E. Coli, Salmonela, Listeria o el Norovirus pueden afectar a los alimentos durante su cosecha o proceso de manipulación. El hecho de que los vegetales provengan de una granja orgánica no es suficiente para descartar la posibilidad de contaminación.
Norovirus obliga a recordar viejos principios de higiene y cocina | lagranepoca.com
Norovirus obliga a recordar viejos principios de higiene y cocina | lagranepoca.com
Con solo lavarse muy bien las manos con agua y jabón, limpiar con esmero las superficie donde se cocina, lavar la ropa con frecuencia y comer alimentos bien cocinados, se pueden evitar un sin número de enfermedades y el bochornoso contagio del norovirus, nefasto cuando se trata de disfrutar unas buenas vacaciones, interrumpidas por más de 24 horas de vómitos y gastroenteritis.
Con solo lavarse muy bien las manos con agua y jabón, limpiar con esmero las superficie donde se cocina, lavar la ropa con frecuencia y comer alimentos bien cocinados, se pueden evitar un sin número de enfermedades y el bochornoso contagio del norovirus, nefasto cuando se trata de disfrutar unas buenas vacaciones, interrumpidas por más de 24 horas de vómitos y gastroenteritis.
Un satellite per le ostriche messicane | Norovirus
Un satellite per le ostriche messicane | attualità | L'espresso food&wine
Dati sul clima, immagini da satellite e elaborazioni al computer potranno presto prevedere lo scoppio di epidemie da norovirus nelle ostriche del Golfo del Messico.
Dati sul clima, immagini da satellite e elaborazioni al computer potranno presto prevedere lo scoppio di epidemie da norovirus nelle ostriche del Golfo del Messico.
lundi 4 juin 2012
Four cases of Legionnaires’ disease confirmed in Edinburgh
Four cases of Legionnaires’ disease confirmed in Edinburgh - Health - Scotsman.com
• Four men between the ages of 39 and 62 have been diagnosed with Legionnaires’ disease
• Four additional people suspected of having disease
• Bacteria is normally contracted via sources of water such as rivers and lakes, but can also be found in man-made water sources such as air conditioning systems
• Four men between the ages of 39 and 62 have been diagnosed with Legionnaires’ disease
• Four additional people suspected of having disease
• Bacteria is normally contracted via sources of water such as rivers and lakes, but can also be found in man-made water sources such as air conditioning systems
Inscription à :
Articles (Atom)